HoTaq DNA Polymerase (hot start)
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Catalog No. |
Size |
Price |
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HT-200 |
1 x 500 U, 5 U/µl |
$100.00 |
|
HT-205 |
5 x 500 U, 5 U/µl |
$400.00 |
|
HT-210 |
10 x 500 U, 5 U/µl |
$740.00 |
|
HT-OEM |
any size, 5 U/ul |
please inquire |
Hot start, chemically modified, patent pending. For Research Use Only.
The product is a chemically modified form of the Taq enzyme. It will be inactive at room temperature, and become active after 10 minutes at 94ºC.
Applications
- Amplification of DNA
- Sequencing ssDNA and dsDNA
- Site-directed mutagenesis
Comparison
Here is the result of comparing MCLAB's HoTaq with some other leading brands.
Recommended storage conditions
:-20°C
Recommended reaction conditions
94°C, 10min. -> (94°C, 10sec. -> 55°C, 30sec. ->72°C, 30sec.) for 25 cycles.
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
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