MCLAB :: Services :: DNA Services :: Standard DNA Sequencing Services

MCLAB DNA Sequencing Services -- the Best Services You Can Intrust!

Testimonials

I'm so glad we found you! We have been raving about your service and your sequencing prowess to everyone in our department, and now it's common knowledge that MCLAB is the best place to go for sample prep and sequencing.

We especially like your plasmid preps from small volumes of cells- it means that we can pick colonies in the morning, have you pick them up in the afternoon and process them, and then we have sequences the next day. Your sequence reads are also amazing- long and accurate! (We also feel lucky to have the free pickup.) The other thing I really like is that you are willing to work with us on new experimental ideas that push the boundaries of sequencing capability.

I can't think of anything that I'm not satisfied with, .......

---Ph.D., Stanford University More >>

Solve Hairpin Problems

Why Choose MCLAB?

1. Commitment: MCLAB is a leader in DNA sequencing services and consumables. We are committed to providing the best services and products to researchers worldwide.

2. Technology: We have proprietary DNA sequencing technologies and consumables to solve hairpin problems and sequence the most difficult templates, including multiple RNAi loops and uneven base distributions.

3. Convenience: We can sequence directly from bacterial colonies or cultures (including 2xYT), phage supernatant, or yeast cells.

4. Large capacity: We can process 10,000+ reactions in 24 hours.

5. Low price: As low as $3.50/reaction (please inquire for details).

6. High quality: Up to 1000 base sequence results using ABI 3730XL sequencers.

7. Fast turnaround time: We can deliver sequencing results within 8 to 24 hours.

8. User friendly: Free repeat, free 100+ universal primers, free pickup (for San Francisco Bay Area), free 96-well alignment tool.

9. Integrated services:We also provide low cost relevant DNA analysis services including DNA fragment analysis, genotyping and the genome sequencing using ABI’s SOLID (coming soon).

How to Use MCLAB DNA Sequencing Services?

For first time orders	1. Email MCLAB to request a quote.
For repeated orders	2. For each order, an order form is required to provide necessary information including template/primer name, billing information, online account (email address) to receive sequencing data, etc. Please download MCLAB's standard DNA sequencing order form, complete it and send it to MCLAB. You can send it through either email attachment (when placing orders by email) or online upload (when placing orders online).
	3. Send your samples to MCLAB (free pick up is provided to San Francisco Bay Area customers), follow Sample Submission Requirements as shown below. If a primer is within our 100+ free universal primers, there is no need to submit it.
	4. Go to the Download Sequences page at MCLAB's secure website after you have received email notification from MCLAB, about the availability of your sequencing results. All of your data files will be listed on that page. For each of them, you can simply click a link to align up to 96 sequences using MCLAB's free tool. On the same page, you can also download a free software called FinchTV to view the chromatogram result files.

Sample Submission Requirements

1. For 96 and more reactions, 96-well PCR plates are required. Otherwise, 8-strip PCR tubes are recommended. For your convenience, MCLAB is now providing related 96-well PCR plate with 8-strip Caps.

2. For separated DNA templates and primers, detailed requirements are shown below:

Template Type	Template Concentration	Template Volume	Primer Concentration	Primer Volume
Plasmid	50 ~ 100 ng/µl	10 µl	5 µM	10 µl
PCR	>10 ng/µl	10 µl	5 µM	10 µl
Cosmid, BAC	1000 ng/µl	10 µl	5 µM	10 µl
Genomic DNA	>1000 ng/µl	10 µl	5 µM	10 µl

3. For mixed DNA templates and primers, detailed requirements are shown below:

Template Type	Total Amount of Template	Total Amount of Primer	Total Volumn (Template+Primer)
Plasmid	500 ng	9 pmol	15 µl
PCR (100-500 bp)	10 ng	9 pmol	15 µl
PCR (500-1000 bp)	20 ng	9 pmol	15 µl
PCR (>1000 bp)	50 ng	9 pmol	15 µl