BDX64
|
Catalog No. |
Size |
Price |
|
BDX-100 |
5 x 0.5 ml |
$210.00 |
|
BDX-101 |
for GC rich, 5 x 0.5 ml |
$210.00 |
|
BDX-OEM |
Any size |
Please inquire |
| Download MSDS Document: MSDS |
For Research Use Only.
Descriptions
MCLAB’s BDX64 is a BigDye enhancing buffer. It has the same ion strength as the BigDye premix 3.1 and 1.1.
- Up to 64 (0.125 µl bigdye in 10 µl reaction) or more fold dilutions by combing with the 5X dilution buffer.
- Enhances the polymerase activity and reduces the extension time from 4 to 1 minute.
- Reduces signal decline rate and results in even peak distribution.
- Optimizes for use with BigDye Chemistry (ABI) on 377, 3100, 3130/3130XL & 3730/3730XL.
An Application Example (64 fold)
- BDX64: 0.875 µl
- BigDye3.1: 0.125 µl
- 5X dilution buffer: 1.5 µl (MCLAB’s catalog # is SBUF-100 for this product)
- Template: 100 ng
- Primer: 3.2 pmole
- H2O: 10 µl
Recommended Cycle Conditions
96°C, 3 min. + 30X (96°C, 10 sec. 50°C, 5 sec. 60°C, 2 min.)
Storage: Store at –20°C
Sequencing Examples
1. Dilution fold 32X:
1.5µl 5X dilution buffer + 0.750µl BDX64 buffer + 0.250µl Bigdye" Terminator 3.1, total reaction volume 10µl
2. Dilution fold 64X:
1.5µl 5X dilution buffer + 0.875µl BDX64 buffer + 0.125µl Bigdye" Terminator 3.1, total reaction volume 10µl
3. Dilution fold 128X:
1.5µl 5X dilution buffer + 0.937µl BDX64 buffer + 0.063µl Bigdye" Terminator 3.1, total reaction volume 10µl
4. Dilution fold 256X:
1.5µl 5X dilution buffer + 0.969µl BDX64 buffer + 0.031µl Bigdye" Terminator 3.1, total reaction volume 10µl
Signal Distributions: before and after BDX64 was added
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