MCLAB :: Human TGF Beta Signaling PCR Array

Human TGF Beta Signaling PCR Array

Catalog No.	Description	Price
hTGFb-102	96-well plate containing 92 pathway regulated genes plus 4 endogenous control genes (2 plates)	$400.00
hTGFb-104	96-well plate containing 92 pathway regulated genes plus 4 endogenous control genes (4 plates)	$800.00
hTGFb-102S	hTGFb-102 with 2.5 ml SYBR Green master mix	$600.00
hTGFb-104S	hTGFb-104 with 5.0 ml SYBR Green master mix	$1200.00

For Research Use Only.

Descriptions

The Transforming growth factor beta (hTGFbeta) signaling pathway is involved in many cellular processes, such as proliferation, apoptosis, differentiation and migration in both the adult organism and the developing embryo and other cellular functions. TGF² family member, which includes structurally related secreted cytokines, such as TGF²s, activins and bone morphogenetic proteins (BMPs). The TGF² signaling pathway is very heavily regulated. There are a variety of mechanisms that the pathway is modulated both positively and negatively: agonists for ligands and R-SMADs; decoy receptors; and ubiquitination of R-SMADs and receptors.

Reaction conditions
95oC, 10min. -> (95oC, 5sec. -> 60oC, 30sec.) for 50 cycles.

Storage Temperature: -20°C

Notes
To achieve accurate quantification, it is highly recommended to do replicates. Also it is important to reduce pipetting error.

Figure:Amplification plot
(A) shows that all genes in the human hTGFbeta signaling pathway were expressed (100% call) our 50 pg of human reference cDNA. The reaction was specific by dissociation curve.(B) and 2% agarose gel (not shown here).

Figure 2: Gene Information