Descriptions

A small hairpin RNA or short hairpin RNA ( shRNA ) is a sequence of RNA that makes a tight hairpin turn that can be used to silence gene expression via RNA interference. ShRNA uses a vector introduced into cells and utilizes the U6 promoter to ensure that the shRNA is always expressed. This vector is usually passed on to daughter cells, allowing the gene silencing to be inherited. The shRNA hairpin structure is cleaved by the cellular machinery into siRNA, which is then bound to the RNA-induced silencing complex (RISC). This complex binds to and cleaves mRNAs which match the siRNA that is bound to it.

The validated shRNA clone collections of MCLAB are mammalian expression vector–based small hairpin RNA (shRNA) clones against genome-wide target genes from human, mouse and any other species. MCLAB provides clones for over-expression of shnRNA of your choice. A region encompassing each shRNA is cloned downstream of a U6 promoter. Upon transfection, the cellular machinery will process the expression of shRNA into mature siRNA and cellular function can be analyzed. A set of four expression constructs for every target gene ensures high knockdown efficiency with minimal off-target effects.

Features

• Pre-designed shRNA with genome wide coverage (human and mouse) that delivers guaranteed successful gene knockdown (>70%).
• Express ShRNA transcripts in their native sequence context to ensure interaction with endogenous processing machinery leading to authentic siRNAs.
• Monitor transduced cells with co-expressed GFP fluorescent marker or firefly Photinus pyralis luciferase . Select cells stably expressing shRNA construct with puromycin selection marker.
• High quality: fully sequenced expression cassettes
• As low as $560 for a kit (4 gene-specific shRNA and two negative controls)
• Convenient: transfection-ready end-toxin free plasmid DNA (5 micrograms per construct)

Kit contents

• 5 micrograms of transfection-ready end-toxin free plasmid DNA in each tube

Storage Temperature:

• -20°C