One-Step^TM Vector-based miRNA Target Screening Systems

MCLAB provides one-step vector-based miRNA target screening systems, which including 2 x PCR master mix, miRNA target screening vector and one-step cloning enzyme mix.

For Research Use Only.

Descriptions

The piReport vector-based miRNA target screening systems are convenient systems for the cloning of possible miRNA target (3’-UTR) for screening in mammalian cells. The piReport vector is prepared by linearized with BamHI and NotI, which greatly improve the efficiency of recombination of a miRNA target (3’-UTR) product into the plasmid by preventing recircularization of the vector and providing a compatible overhang for recombination of miRNA (3’-UTR) products generated by any thermostable polymerases. A double digestion may be used to release the insert from the vector.

The piReport Vector-based miRNA target screening systems contain linearized miRNA target screening vector piReport/EmGFP or piReport/hluc, 2 x PCR master mix for PCR amplifying miRNA targets, High efficiency (>1 x 10⁹ ) DH5a Competent Cells, one-step^TM cloning/recombination enzyme mix.

Features

• The cloning systems include the PCR 2x master mix, and makers either EGFP or Fire fly luciferase
• Clone any miRNA target into the vector you choose
• No restriction digestion, phosphatase treatment, or ligation required
• Simple 45 min single-tube reaction on ice protocol
• High Efficiency with >=98% positive clones
• High throughput application

Kit contents

• Box 1: 2 x PCR master mix
• Box 2 : One-step cloning enzyme mix with piReport/EmGFP or piReport/hluc
• Box 3 : High efficiency (>1 x 10⁹) Chemical Competent E. Coli DH5a cells

Storage Temperature:

• Box 1 : -20°C
• Box 2 : -20°C
• Box 3 : -80°C