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X-Gal Solution

The β-galactosidase substrate X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic stain for β-gal activity, and is commonly used to distinguish between recombinants and nonrecombinants by lacZ α-complementation with appropriate vectors and hosts. X-Gal Solution is provided as a convenient 20 mg/ml concentrate in DMSO, ready for dilution to culture medium or appropriate buffers.

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