Description:
The custom 5’ adenylation service is designed for generating 5’ pre-adenylated DNA/RNA oligonucleotides. Adenylated oligos with a pyrophosphate linkage are substrates of T4 RNA ligase in the absence of ATP, which can significantly reduce undesired self-ligation and other side products.

Application:
5’ adenylated oligos are used in sequence detection, sequence capture, sequencing by ligation and attachment of adapters (e.g. RNA library preparation for NGS and miRNA library construction), etc.

Starting material requirement:
1)    Order oligos with 5’ phosphate.
2)    To prevent a 5’ adenylated oligo from self-ligating, a blocking group is required on the 3’ end, such as a 2’, 3’-Dideoxynucleotide or 3’ (C3) Propyl Spacer.
3)    5’ adenylated oligos require an extra purification after the adenylation step and are not compatible with heat-sensitive modifications. Prices are valid for oligonucleotides up to 30 bases. For longer oligos, please inquire for a custom quotation.