5´ DNA Adenylation Kit
$90.64 – $339.90
5´ DNA Adenylation Kit is for the enzymatic synthesis of 5’ adenylated ssDNA linkers.
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| APPK-100 | 10 reactions,50 uM | $90.64 | |||
| APPK-200 | 50 reactions,50 uM | $339.90 |
- Description
- Additional information
- Documents
- Q&A
Description
Description:
5´ DNA Adenylation Kit is for the enzymatic synthesis of 5’ adenylated ssDNA linkers. The kit is optimized to produce the adenylated DNA with or without 3´ terminator. The 5´ DNA adenylation kit can simply and efficiently generate greater than 95% conversion of pDNA to AppDNA.
Features:
- One step reaction. Simple and efficient.
- 65°C reaction temperature reduces secondary structural concerns.
- Highly efficient process eliminates the need for purification of the product.
- Easily scale up from pmol to µmol range.
Application:
Enzymatic 5´adenylation of single-stranded DNA linkers for next generation sequencing (NGS).
Kit Components:
10X 5’ DNA Adenylation Reaction Buffer
500 mM Sodium Acetate (pH 6.0 @ 25°C)
100 mM MgCl2
50 mM DTT
1 mM EDTA
1 mM ATP
Mth RNA Ligase
Storage Temperature:-20°C
Additional information
| OPTIONS | 10 reactions,50 uM, 50 reactions,50 uM |
|---|
Manual & Protocols
The Q&A for this product will be available soon.
