50% Triton X-114
50% Triton X-114

TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C). At temperatures above the cloud point, detergent solutions separate into aqueous and detergent-enriched phases. This makes Triton X-114 particularly useful in separating lipophilic proteins from hydrophilic proteins.2,3 For example, HMG-CoA reductase was recovered quantitatively in the aqueous phase of a biphasic system formed by TRITON X-114 at 30 °C.
 
Name Cat# Description Price Qty
50% Triton x-114
50TX114-1L
50%, 1L
$67.98
50% Triton x-114
50TX114-1GAL
50%, 1GAL
$169.95

Description:
TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C). At temperatures above the cloud point, detergent solutions separate into aqueous and detergent-enriched phases. This makes Triton X-114 particularly useful in separating lipophilic proteins from hydrophilic proteins.2,3 For example, HMG-CoA reductase was recovered quantitatively in the aqueous phase of a biphasic system formed by TRITON X-114 at 30 °C.


References:

  1. Helenius, A., and Simons, K., Biochim. Biophys. Acta, 415, 29-79 (1975).
  2. Bordier, C., J. Biol. Chem., 256, 1604-1607 (1981).
  3. Neugebauer, J., Meth. Enzymol, 182, 247-249 (1990).
  4. Concepcion, J.L. et al., Arch. Biochem. Biophys., 352, 114-120 (1998).
  5. Gu, T., and Galera-Gomez, P.A., Colloids and Surfaces A: Physicochem. Eng. Aspects, 104, 307- 312 (1995).
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