
50% Triton X-114
TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C). At temperatures above the cloud point, detergent solutions separate into aqueous and detergent-enriched phases. This makes Triton X-114 particularly useful in separating lipophilic proteins from hydrophilic proteins.2,3 For example, HMG-CoA reductase was recovered quantitatively in the aqueous phase of a biphasic system formed by TRITON X-114 at 30 °C.
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