Description:
TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C). At temperatures above the cloud point, detergent solutions separate into aqueous and detergent-enriched phases. This makes Triton X-114 particularly useful in separating lipophilic proteins from hydrophilic proteins.^2,3 For example, HMG-CoA reductase was recovered quantitatively in the aqueous phase of a biphasic system formed by TRITON X-114 at 30 °C.

References:

1. Helenius, A., and Simons, K., Biochim. Biophys. Acta, 415, 29-79 (1975).
2. Bordier, C., J. Biol. Chem., 256, 1604-1607 (1981).
3. Neugebauer, J., Meth. Enzymol, 182, 247-249 (1990).
4. Concepcion, J.L. et al., Arch. Biochem. Biophys., 352, 114-120 (1998).
5. Gu, T., and Galera-Gomez, P.A., Colloids and Surfaces A: Physicochem. Eng. Aspects, 104, 307- 312 (1995).