All-In-One 5X Reverse Transcription Mix Kit
All-In-One 5X Reverse Transcription Mix Kit

MCLAB's All-In-One 5X Reverse Transcription Mix Kit is optimized to synthesize the first strand cDNA for RT-PCR and RT-qPCR.

 
Name Cat# Description Price Qty
All-In-One 5X Reverse Transcription Mix Kit
5AIO-100
25 rxn(4ul/rxn),100 ul
$112.17
All-In-One 5X Reverse Transcription Mix Kit
5AIO-200
100 rxn(4ul/rxn),400 ul
$395.42

Description
MCLAB's  All-In-One 5X Reverse Transcription Mix Kit is optimized to synthesize the first strand cDNA for RT-PCR and RT-qPCR. MCLAB's QuantumScript™ HD Reverse Transcriptase is an engineered mutation with increased sensitivity, improved specificity, maximum thermostability and reduced RNase H activity. The optimal fist-strand cDNA synthesis temperature for this enzyme is 50°C. At higher temperatures the reverse transcription reaction improves cDNA yields for difficult RNA transcription. This Pre-mixed 5X concentrated solution includes all necessary components for the first-strand synthesis: 5X buffer, dNTPs, MgCl2, DTT, primers (optimized mixture oligo (dT) and random primers), RNase inhibitors, QuantumScript™ HD Reverse Transcriptase, It will simplify your first strand cDNA synthesis process. 

Features

  • Easy-to-use pre-mixed package
  • High fidelity, yield and sensitivity
  • Broader linear range
  • Complete cDNA synthesis guaranteed
  • Maximum sample volume available
  • Robust and high throughput  
  • Works with difficult sequences

Application
RNA reverse transcription 

Recommended Storage Condition
-20 °C

Shipping Condition
dry ice

RNAse Inhibitor
Figure 1. cDNA was synthesized in 20 µl for 30 minutes from 100 ng total RNA with different 5x All in one master mix. 1 ul of cDNA was used for qPCR, The qPCR was performed in MClab 2x HotSybr qPCR system.

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