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BL21 Competent <i>E. coli</i>
BL21 Competent E. coli

Chemically competent E. coli cells suitable for transformation and protein expression. This strain does not express the T7 RNA polymerase.
 
Name Cat# Description Price Qty
bl21 Competent E. Coli
BL21-100
10x100μl (10 tubes), >1x10⁸
$110.00
bl21 Competent E. Coli
BL21-196
96x50μl (96-tube rack), >1x10⁸
$320.00

Description:
Chemically competent E. coli cells suitable for transformation and protein expression. This strain does not express the T7 RNA polymerase.


Application:

  • Ideal for expressing proteins in E. coli

Recommended torage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

Recommended reaction conditions:

  • Mix DNA with competent cells
  • Let stand on bench for 5 minutes
  • Direct plate

Genotype:
E. coli B F- dcm ompT hsdS(rB- mB-) gal [malB+]K-12(λS)

Reference:
Miroux B, Walker JE. J. Mol. Biol. (1996) 260, 289-298.


Competent Cell Type:
Chemically

Transformation Efficiency (cfu/μg):
>1x108

Blue-Whiet Screening:
No

Strain:
B

Reduces Recombination:
No

Cloning Methylated DNA:
No

Improves Plasmid Quality:
No

Preparing Unmethylated DNA:
Not suitable

T1 Phage Resistant:
No

RecA Deficient:
No

Manual & Protocols



COA ( Certificate of Analysis )



MSDS & Certificates

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