Description
High yields, high efficiency, and no heat shock necessary.

Application

• Ideal for expressing proteins in E. coli.
• BL21(DE3) cells carry the lambda DE3 lysogen.

Recommended torage condition
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

Recommended reaction conditions

• Mix DNA with competent cells
• Let stand on bench for 5 minutes
• Direct plate

Genotype
F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

Reference
Miroux B, Walker JE. J. Mol. Biol. (1996) 260, 289-298.

Competent Cell Type:
Chemically

Transformation Efficiency (cfu/μg):
>1x10⁸

Blue-Whiet Screening:
No

Strain:
B

Reduces Recombination:
No

Cloning Methylated DNA:
No

Improves Plasmid Quality:
No

Preparing Unmethylated DNA:
Not suitable

T1 Phage Resistant:
No

RecA Deficient:
No