BigDye<sup>®</sup> Terminator 5X Sequencing Buffer
BigDye® Terminator 5X Sequencing Buffer

The BigDye® Terminator 5X Sequencing Buffer reduces sequencing costs without affecting sequence accuracy or read length. Its buffer system enables to use less dye terminator mix.
           
MSDS          
MSDS          
 
Name Cat# Description Price Qty
BigDye Terminator 5X Sequencing Buffer
SBUF-100
1 ml
$25.75
BigDye Terminator 5X Sequencing Buffer
SBUF-110
28 ml
$484.10
BigDye Terminator 5X Sequencing Buffer
SBUF-120
233 ml
$3599.85
BigDye Terminator 5X Sequencing Buffer
SBUF-OEM
Any size
please inquire

Description:
The BigDye® Terminator 5X Sequencing Buffer reduces sequencing costs without affecting sequence accuracy or read length. Its buffer system enables to use less dye terminator mix.

Applications:

  • DNA sequencing
  • Fragment analysis

Recommended Storage Condition: 4°C

Recommended Protocol:
Prepare sequencing reactions (10µl rxn) according to the following:
- Add dye terminator mix 0.25-0.5µl
- Add 5X Sequencing Buffer 2µl
- Add template (100-200 ng/µl) 1 µl
- Add primer (3 pmol/µl) 1 µl
- Add H2O to 10 µl
Cycle the reaction according to the following protocol:

Step 1: 96°C  3 minutes
Step 2: 96°C  10 seconds
Step 3: 50°C  5 seconds
Step 4: 60°C  2 minutes and 30 seconds
(25 to 30 cycles from step 2 to step 4)
Step 5: 4°C hold

Notes:
Other cycle conditions may work well for individual users. These conditions work well for most of the samples processed in our lab using reduced volume and reduced terminator mix concentrations.

The Q&A for this product will be available soon.
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