Streptavidin is a non-glycosylated, 52.8 kDa, tetrameric bacterial protein isolated from Streptomyces avidinii with a very high affinity for biotin. Streptavidin-biotin bond is the strongest known non-covalent interaction with Kd ~10-15 M. Because streptavidin lacks any carbohydrate modification and has a near-neutral pI, it has the advantage of much lower nonspecific binding than avidin.
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