BirA Biotin-protein Ligase
$0.00 – $555.17
Biotin-protein ligase (EC 6.3.4.15) activates biotin to form biotinyl 5′ adenylate and transfers the biotin to biotin-accepting proteins. It also functions as a biotin operon repressor.
| SKU | OPTIONS | Price | Quantity | ||
|---|---|---|---|---|---|
| BBLA-100 | BirA Biotin-protein Ligase (100 ug,1 mg/ml) | $79.31 | |||
| BBLA-200 | BirA Biotin-protein Ligase (1 mg,1 mg/ml) | $555.17 | |||
| BBLA-OEM | BirA Biotin-protein Ligase (Any size) | Please inquire | |||
| BBLA-110 | BirA Biotin-protein Ligase(His+) (100 ug,1 mg/ml) | $79.31 | |||
| BBLA-210 | BirA Biotin-protein Ligase(His+) (1 mg,1 mg/ml) | $555.17 | |||
| BBLAH-OEM | BirA Biotin-protein Ligase(His+) (Any size) | Please inquire |
- Description
- Additional information
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Description
Description
Biotin-protein ligase (EC 6.3.4.15) activates biotin to form biotinyl 5′ adenylate and transfers the biotin to biotin-accepting proteins. It also functions as a biotin operon repressor. The protein is encoded by the birA gene. Other names for this enzyme include: biotin ligase; biotin operon repressor protein; birA; biotin holoenzyme synthetase; biotin-(acetyl-CoA carboxylase) synthetase.
Applications
- Western blot
- Functional Studies of enzyme kinetics
- Screening inhibitors and selectivity profiling
Source
E. coli
Molecule Weight
The recombinant Biotin-protein ligase consists of amino acids 2-321 and has a predicted molecular mass of 37 kDa.
Purity
> 95 % as determined by SDS-PAGE
Activity: 5,000 Units/µg
Definition of Activity
1 Unit is the amount of enzyme that will biotinylate 1 pmol of peptide substrate in 30 minutes at 30°C using the reaction buffers provided and 40 µM peptide substrate*.
*The peptide substrate used in the enzyme assays was a 27kDa AviTag’d recombinant protein expressed by Pichia pastoris.
Contaminating proteases: <0.01% as chymotrypsin-like activity
Storage: -80 Avoid repeated freeze-thaw cycles.
Storage Buffer
50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 1 mM DTT, and 50% Glycerol
Shipping: Dry ice
Reference
1. Schatz, P. (1993) Biotechnology 11, 1138-1143
Additional information
| OPTIONS | BirA Biotin-protein Ligase (100 ug,1 mg/ml), BirA Biotin-protein Ligase (1 mg,1 mg/ml), BirA Biotin-protein Ligase (Any size), BirA Biotin-protein Ligase(His+) (100 ug,1 mg/ml), BirA Biotin-protein Ligase(His+) (1 mg,1 mg/ml), BirA Biotin-protein Ligase(His+) (Any size) |
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Manual & Protocols
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