Cas13a (CRISPR Associated Protein 13a)
Cas13a (CRISPR Associated Protein 13a)

Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic
repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. Cas13a, (previously referred to as C2c2) is part of the Type VI CRISPR-Cas system.
 
Name Cat# Description Price Qty
Cas13 a
CAS13a-100
100 μg,1 mg/ml
$199.00
Cas13 a
CAS13a-200
1 mg,1 mg/ml
$990.00
Cas13 a
CAS13a-300
100 mg,10 mg/ml
please inquire
d Cas13 a
dCAS13a-100
100 μg,1 mg/ml
$199.00
d Cas13 a
dCAS13a-200
1 mg,1 mg/ml
$990.00
d Cas13 a
dCAS13a-300
100 mg,10 mg/ml
please inquire
Cas13 d
CAS13d-100
100 μg,1 mg/ml
$199.00
Cas13 d
CAS13d-200
1 mg,1 mg/ml
$990.00
Cas13 d
CAS13d-300
100 mg,10 mg/ml
please inquire
d Cas13 d
dCas13d-100
100 μg,1 mg/ml
$199.00
d Cas13 d
dCas13d-200
1 mg,1 mg/ml
$990.00
d Cas13 d
dCas13d-300
100 mg,10 mg/ml
please inquire

Description
Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. Cas13a, (previously referred to as C2c2) is part of the Type VI CRISPR-Cas system. Cas13a functions similarly to Cas9, using a ~64-nt guide RNA to encode target specificity. The Cas13a protein complexes with the guide RNA via recognition of a short hairpin in the crRNA, and target specificity is encoded by a 28 – 30-nt spacer that is complementary to the target region. Cas13a cuts within the crRNA direct repeat in a pre-crRNA array to produce a mature Cas13a-crRNA complex.

In addition to programmable RNase activity, all Cas13s exhibit collateral activity after recognition and cleavage of a target transcript, leading to non-specific degradation of any nearby transcripts regardless of complementarity to the spacer. The RNA-activated RNA cleavage activity of Cas13a allows for RNA detection and potential diagnostic applications. Catalytically inactive versions of Cas13 may also be useful as programmable RNA binding proteins to regulate or image specific transcripts.

Cas13a protein is highly purified with no detectable RNAse and DNAse activity. The dcas13a is a mutant cas13a. It can bind target RNA and can not cut the RNA. Cas13d is from Ruminococcus flavefaciens(casRX) and it has 94KD. The dcas13d has mutants which can not cut the target RNA but binds.

Applications
• Targeted RNA knockdown
• RNA editing
• Nucleic acid detection

Source
E. coli

Organism
Leptotrichia wadeii (strain F0279)

UniProt #
U2PSH1

Molecule Weight
The mature recombinant Cas13a is a 143.7 kDa protein containing 1182aa.

Purity
> 95 % as determined by SDS-PAGE

Storage
-80 Avoid repeated freeze-thaw cycles.

Cas13a Storage Buffer
0.3 M NaCl
20 mM Tris-HCl, pH 8.0
0.5 mM EDTA
1 mM DTT
50% Glycerol

10X Cas13a Reaction Buffer
200 mM HEPES, pH 6.8
90 mM MgCl2

 

COA ( Certificate of Analysis )



MSDS & Certificates

The Q&A for this product will be available soon.
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