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Description Adaptive immune systems from prokaryotes utilize clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR associated (Cas) proteins to cleave foreign genetic material. CasRx, Cas13d from Ruminococcus flavefaciens XPD3002, was highly active and short, 112KD. In addition to programmable RNase activity, all Cas13d exhibit collateral activity after recognition and cleavage of a target transcript, leading to non-specific degradation of any nearby transcripts regardless of complementarity to the spacer. Applications • Targeted RNA knockdown • RNA editing • Nucleic acid detection Source E. coli Organism Ruminococcus flavefaciens XPD3002 (CasRx), Molecule Weight: 112KD Purity > 95 % as determined by SDS-PAGE Storage -20 °C. Avoid repeated freeze-thaw cycles. Cas13 Storage Buffer 0.3 M NaCl 20 mM Tris-HCl, pH 8.0 0.5 mM EDTA 1 mM DTT 50% Glycerol 10X Cas13 Reaction Buffer 200 mM HEPES, 50 mM MgCl2, 1 M NaCl, 1 mM EDTA, pH 6.5 10X Cas13 sherlock reaction buffer (requested) 200 mM HEPES, 90 mM MgCl2, pH 6.8 |
COA ( Certificate of Analysis ) |
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| Polylysine Magnetic Beads | dCas13d | TtAGO (Tth Argonaute) |
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$50.00
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$100.00
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$100.00
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| Cas12a2 Nuclease | Nucleocapsid Protein (N-protein) | Recombinant Proteinase K, Solution or Lyophilized Powder |
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$100.00
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$100.00
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$100.00
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| dCas13a | PfAGO (Pfu Argonaute) | Cas12a Nuclease |
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$100.00
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$100.00
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$100.00
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