Cloning & Sub Cloning

Cloning & Sub Cloning

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Choo-Choo Cloning™ Kits
Choo-Choo CloningTM Kits are highly efficient directional PCR cloning kits for rapid, ligase and restriction enzyme-independent PCR cloning.

Important Note: The Choo-Choo Blue Chemical Competent E. coli cells are the same as Dh5-Alpha competent E. coli (DA-100).

 
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Choo-Choo Cloning ready vectors
This linearized vector does not carry any tag sequence. The Cloning / expression region of the coding strand is shown in the PDF links. Sequencing primers for final confirmation is CMV promoter or T7 promoter and SV40 poly A reverse or SP6 promoter.
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Ab Cloning 5'-RACE Kit

The Ab cloning RACE kit provides a novel method for performing both 5' and 3’ rapid amplification of cDNA ends (RACE) with optimized primers for cloning both of heavy and light chain of human and mouse antibodies.

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All-In-One 5X Reverse Transcription Mix Kit

MCLAB All in one 5X Reverse Transcription Mix Kit is optimized to synthesize the first strand cDNA for RT-PCR and RT-qPCR.

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First Strand cDNA Synthesis Kit
First Strand cDNA Synthesis Kit can be used to synthesize the first strand cDNA.
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