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Chemically competent E. coli cells suitable for transformation and protein expression. This strain does not express the T7 RNA polymerase.
High yields, high efficiency, and no heat shock necessary.
High yields, high efficiency, and no heat shock necessary.
High transformation efficiency (>109) with minimum order of $550.00
The DH10B competent E. coli has a transformation efficiency of 1 x 109 cfu/µg for supercoiled DNA and is ideal for high-efficiency cloning and plasmid propagation. It allows stable replication of high-copy number plasmids. In comparison with DH5a, methylated DNA from genomic preparation could be efficiently transformed into DH10B, therefor, the DH10B cells are more applicable than DH5a in generating genomic libraries containing methylated cytosine or adenine residues.
DH5a™ is the most frequently used E. coli strain for routine cloning applications.
High yields, high efficiency, no heat shock step.
High yields, high efficiency, no heat shock necessary.
Chemically competent E. coli cells with high stability suitable for high efficiency transformation.
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