Description:
The MCLAB's DNA dA-Tailing Kit is used to add an "A" base to the 3´ end of a blunt phosphorylated DNA fragment. This treatment creates compatible overhangs for the next step of DNA sample preparation. The kit has been optimized to the maximum efficiency and convenience in DNA sample preparation workflow for next-generation sequencing.

Function:
The superior function of MCLAB's DNA dA-Tailing Kit depends on our proprietary enzyme systems. Unique improvement to the key enzyme increases sensitivity, flexibility and speed of next-generation sequencing. Through the MCLAB's DNA dA-Tailing Kit, a dAMP can be added to the 3´ end of an end repaired blunt DNA fragment. This prepares the DNA fragment for efficient ligation to the adapters or cloning vectors with a single "T" base overhang at their 3’ ends, and effectively prevents insert-to-insert ligation as well.

Features:
- Simplified workflow, reducied hands-on time
- Optimized reaction conditions, increased sensitivity
- Up to 10µg end repaired blunt DNA
- Automation friendly format

Components:
Sufficient reagents are supplied in the MCLAB DNA dA-Tailing Kit to process 20 or 100 DNA samples.

DNA dA-Tailing Kit (20 reactions):
dA-Tailing Enzyme: 20µl
dA-Tailing Buffer: 200µl

DNA dA-Tailing Kit (100 reactions):
dA-Tailing Enzyme: 100µl
dA-Tailing Buffer: 1000µl

Recommended Storage Condition: -20°C.