E. coli SSB

$0.00$453.20

Single-Stranded DNA Binding Protein (SSB) preferentially binds single-stranded DNA, forming a tetramer of four identical 18.9 kDa subunits which protects 8-16 nucleotides.
SKU OPTIONS PriceQuantity
ESSB-100 1.25 mg (5.0 mg/ml) $141.63
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ESSB-200 5.0 mg (5.0 mg/ml) $453.20
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ESSB-OEM Any Size Please inquire
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Description

Description:
Single-Stranded DNA Binding Protein (SSB) preferentially binds single-stranded DNA, forming a tetramer of four identical 18.9 kDa subunits which protects 8-16 nucleotides, while not binding well to double-stranded DNA. In nature, SSB participates in DNA replication, recombination, and repair functions. In vitro, SSB has been found to stimulate certain DNA polymerase-mediated reactions by relaxing DNA secondary structure and enhancing enzyme processivity.

Applications:

  • Effective in fluorescence polarization assays
  • Eliminates pausing when sequencing through strong secondary structures
  • Helps obtain longer read lengths in pyrosequencing for SNP analysis

Source:
A recombinant E. coli strain carrying the E. coli SSB gene

Supplied in:
50 mM Tris-HCl
200 mM NaCl
1.0 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C

Recommended Storage Condition: -20°C

Additional information

OPTIONS

1.25 mg (5.0 mg/ml), 5.0 mg (5.0 mg/ml), Any Size

Manual & Protocols

COA ( Certificate of Analysis )

MSDS & Certificates

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