
Description: The MCNext™ SYBR® Fast qPCR Library Quantification Kit provides a fast and reliable solution to determine the library concentration with a direct estimate cluster density. This direct measurement method significantly saves time and resources comparing with other qPCR library quantification kits on the market. This is achieved by providing a constructed library rather than a single DNA fragment as a control standard with known cluster generation density on Illumina® sequencing platforms. The MCNext™ SYBR® Fast qPCR Library Quantification Kit utilizes MCLAB’s high performance fast PCR enzyme by a Phi X genome based Library Standards (for seven 10-fold dilutions) with an average size of 570 bp, providing quick and accurate quantification with 0.0001 – 100 pM dynamic range in 60 min. Based on the quantification, the result could be directly converted to cluster numbers for loading volume references. The MCNext™ SYBR® Fast qPCR Library Quantification Kit is a rapid solution for your library construction QC to add value to your workflow and increase confidence in your results. The proprietary Phi X Library Standards consist of a group of Illumina® sequencing adapter-ligated DNA fragments derived from the Phi X genome with well-characterized sequence and well-balanced GC content (50%). To use this library as a control for libraries constructed for sequencing on Illumina® sequencing platforms is far superior than a single DNA fragment as control standard used by other qPCR quantification kits on the current market. It can be rapidly interpolated for the estimating cluster density and serves as a quantification baseline for sample libraries without the need to have another sequencing run for a cluster density measurement purpose. It is an excellent control with measured cluster generation conversion parameter to examine the library quality and quantity, allows you to quickly determine if an error is related to the sample preparation before a high-cost sequencing run. Quantification is fast, direct and accurate by extrapolation against the standard curve generated using the seven 10-fold Library Standards dilutions, followed by a simple cluster number conversion. The MCNext™ SYBR® Fast qPCR Library Quantification Kit is available in 3 types (Regular ROX, Low ROX and Universal) based on the internal reference dye preferences of the PCR thermocyclers. The packages include ready-to-use 2X Master Mix, 10X Primer Mix and Phi X Library Standards. Features The MCNext™ SYBR® Fast qPCR Library Quantification Kit provides researchers with an accurate and sensitive method for quantifying NGS libraries.
Convenient packages for all types of real time PCR platforms Kit Components: MCNext™ SYBR® Fast qPCR Library Quantification Kit (Universal) (IQPQ-UN)
MCNext™ SYBR® Fast qPCR Library Quantification Kit (Low ROX) (IQPQ-LR)
MCNext™ SYBR® Fast qPCR Library Quantification Kit (Regular ROX) (IQPQ-RR)
Storage Condition: -20°C Shipping Condition: Dry ice |
Manual & ProtocolsCOA ( Certificate of Analysis )MSDS & Certificates |
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