Description:

The error rate of gene synthesis is 1 out of 1000~1500 nt, which is a major challenge to make long gene fragments. With the high-throughput sequencing platform and the proprietary technologies, MCLAB offers a high quality and cost-effective Gene Assembly Service for custom designed, error-free and long double-stranded DNA fragments (up to >10kb), as a PCR product or inserted into our assembly vector. The system is very robust and highly efficient with 100% accuracy guaranteed.

Features:

• Subcloning of gene blocks (up to 2kb) into assembly vector and Screening for error-free clones by High-Throughput Sanger Sequencing.
• Assembly of gene blocks into long DNA fragments (up to >10kb) and verify the sequences .
• Optimized Gene Synthesis Design for difficult oligos, repetitive sequences, toxic sequences and long fragments.
• Delivery of sequence-verified gene fragments as PCR products or as an insert in the assembly vector.
• Flexibility sequence-verified gene fragments can be subcloned into a compatible vector of your choice.
• Quick turnaround time within 7 business days for gene fragments < 2kb.

Applications:

• Constructs for protein expression and purification
• Donor constructs for gene editing experiments
• gRNA cassettes for Cas9-based gene editing
• Template for in vitro transcription
• Applications in bioengineering and synthetic biology

Note:

• For difficult sequences, e.g., highly repetitive sequences, high GC contents, toxic sequences, etc.,please inquire.
• An additional fee ($350) applies to subcloning.