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Gene Assembly Service
Gene Assembly Service

With the high-throughput sequencing platform and the proprietary technologies, MCLAB offers a high quality and cost-effective Gene Assembly Service for custom designed, error-free and long double-stranded DNA fragments (up to >10kb), as a PCR product or inserted into our assembly vector.
 
Name Cat# Description Price Qty
Gene Assembly Service
GA2kb
Gene Assembly < 2kb
$300.00
Gene Assembly Service
GA4kb
Gene Assembly 2-4kb
$600.00
Gene Assembly Service
GA6kb
Gene Assembly 4-6kb
$1000.00
Gene Assembly Service
GA8kb
Gene Assembly 6-8kb
$1500.00
Gene Assembly Service
GA10kb
Gene Assembly 8-10kb
$2000.00
Gene Assembly Service
GANkb
Gene Assembly > 10kb
please inquire

Description:

The error rate of gene synthesis is 1 out of 1000~1500 nt, which is a major challenge to make long gene fragments. With the high-throughput sequencing platform and the proprietary technologies, MCLAB offers a high quality and cost-effective Gene Assembly Service for custom designed, error-free and long double-stranded DNA fragments (up to >10kb), as a PCR product or inserted into our assembly vector. The system is very robust and highly efficient with 100% accuracy guaranteed.

GeneAssembly-002.png

Features:

  • Subcloning of gene blocks (up to 2kb) into assembly vector and Screening for error-free clones by High-Throughput Sanger Sequencing.
  • Assembly of gene blocks into long DNA fragments (up to >10kb) and verify the sequences .
  • Optimized Gene Synthesis Design for difficult oligos, repetitive sequences, toxic sequences and long fragments.
  • Delivery of sequence-verified gene fragments as PCR products or as an insert in the assembly vector.
  • Flexibility sequence-verified gene fragments can be subcloned into a compatible vector of your choice.
  • Quick turnaround time within 7 business days for gene fragments < 2kb.

Applications:

  • Constructs for protein expression and purification
  • Donor constructs for gene editing experiments
  • gRNA cassettes for Cas9-based gene editing
  • Template for in vitro transcription
  • Applications in bioengineering and synthetic biology

Note:

  • For difficult sequences, e.g., highly repetitive sequences, high GC contents, toxic sequences, etc.,please inquire.
  • An additional fee ($350) applies to subcloning.
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