Glutathione Agarose CL-6B is based on highly cross-linked agarose 6% beads which have structural characteristics that result in superb physical strength, excellent flow properties, low backpressure, and an open pore structure. Glutathione Agarose CL-6B allows operation at very high flow rates, resulting in increased throughput and rapid column cleaning between chromatography runs.
| Chromatography technique |
GST-tagged protein purification |
| Matrix |
Highly cross-linked 6% beaded agarose |
| Active group |
Glutathione |
| Active group density |
>40 umol/ml drained medium |
| Spacer |
1,4-bis(2,3-epoxypropoxy)butane (12 atom stable and uncharged ether hydrophilic linkage) |
| Bead geometry & size |
Spherical 50 to 150 um |
| Bead mean diameter d50v |
90 um |
| Linear flow velocity |
<75 cm/h at 25 degC, HR 16/10 column, 5 cm bed height |
| Recommended linear flow rate |
<30 cm/h |
| Pressure/flow specification |
Base matrix 100-200 cm/h, pressure drop cm H2O/bed height = 15, bed height 10 cm, 5 cm i.d. |
| 1ml Cartridge bed dimensions |
7×25 mm |
| 1ml Cartridge bed height |
25 mm |
| 1ml Cartridge bed volume |
1 ml |
| 1ml Cartridge column I.D |
7 mm |
| 5ml Cartridge bed dimensions |
16×25 mm |
| 5ml Cartridge bed height |
25 mm |
| 5ml Cartridge bed volume |
5 ml |
| 5ml Cartridge column I.D |
16 mm |
| Maximum cartridge flow rate |
5 ml/min (1 ml cartridge) or 20 ml/min (5ml cartridge) |
| Recommended cartridge flow rate |
1 ml/min (1 ml cartridge) or 5 ml/min (5 ml cartridge) |
| Maximum pressure during operation |
5 bar [0.5 MPa] (70 psi) |
| pH Stability working range |
3 to 13 |
| pH Stability cleaning in place (cip) |
2 to 14 |
| Chemical stability |
Stable to commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride, Stable in organic solvents, e.g. 50% methylformamide and 50% dioxane. |
| Storage |
2 to 8 degC |
