Description:
High stability, high efficiency, no heat shock step. For Research Use Only.

Applications:

• Ideal for sub-cloning and scale-up applications
• HB101 strain is a hybrid K12 x B bacterium, containing the recA13 mutation that minimizes recombination and aidsin insert stability. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening. Transform efficiency is around 1.0x10⁷ ~ 1.0x10⁹ cfu/µg with pUC18 control DNA.

Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

Recommended reaction conditions:

• Mix DNA with competent cells
• Let stand on bench for 5 minutes
• Direct plate

Genotype:
F- mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ-

Reference

1. Boyer, H. W., Roulland-Dussoix, D. J. Mol. Biol., (1969) 41: 459-472

Competent Cell Type:
Chemically

Transformation Efficiency (cfu/μg):
>1x10⁹

Blue-Whiet Screening:
No

Strain:
Hybrid

Reduces Recombination:
Yes

Cloning Methylated DNA:
Yes

Improves Plasmid Quality:
Yes

Preparing Unmethylated DNA:
Yes

T1 Phage Resistant:
No

RecA Deficient:
Yes