The MCLAB's HoTaq Taqman probe One-step Real-time RT-PCR Kit offers a unique system for performing probe based realtime RT-PCR in a single step within a single tube with an optimized reaction condition, which utilizes our own proprietary engineered QuantumScript HD reverse transcriptase and hot-start Taq DNA Polymerase with high purity . No additional reagents or steps are required once the reaction is initialized. This novel kit enables to quantitatively detect specific RNA targets with high sensitivity, unparalleled convenience, and a wide dynamic range.
Description:
List of Components: Sufficient reagents are supplied in the MCLAB's HoTaq One-step Real-time RT-PCR Kit for 40 or 200 rxns. Upon receipt of the kit, immediately store the components at –20 °C in a freezer without a defrost cycle. It is recommended to prevent light exposure as little as possible. 40 rxns: 2x RT-PCR Buffer*: 1.5ml x 1 25x RT-PCR Enzyme Mix: 40 ul x1 200 rxns: 2x RT-PCR Buffer*: 7.5ml x 1 25x RT-PCR Enzyme Mix: 200 ul x1 * Contains regular level of ROX, dNTPs and optimized buffer components for Real-time PCR machines ABI 7000, 7300, 7700, 7900 and Stratagene Mx 3000P, Mx 3005P. Additional Materials Required: The following reagents, instruments and consumables are supplied by the user:
Reference:
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Manual & ProtocolsCOA ( Certificate of Analysis )MSDS & Certificates |
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