Human and Mouse Housekeeping Gene Primer Sets

$22.61$90.59

manuals_protocols                             manuals_protocols

Protocol for human      Protocol for mouse

SKU: HKG Categories: ,
SKU OPTIONS PriceQuantity
hHKG-100 2 human genes, h18S rRNA and hActß, 100rxns $22.61
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hHKG-110 2 high expression level hACtß, hGapdh; 2 medium expression level genes, hHprt1, hTfrc; 2 low expression level genes, hGusb, hUbc; 100rxns $56.60
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hHKG-120 All 12 human housing keeping genes at different expression levels, 100rxns $90.59
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mHKG-100 2 mouse genes, m18S rRNA and mActß, 100rxns $22.61
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mHKG-110 2 high expression level mACtß, mGapdh; 2 medium expression level genes, mHprt1, mHsp90ab1; 2 low expression level genes, mGusb, mTbp; 100rxns $56.60
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mHKG-120 All 12 mouse housekeeping genes at different expression levels, 100rxns $90.59
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Description

Table: Validated primer sets for human and mouse housekeeping genes and their expression levels.

Human Genes
Mouse Genes
Name
GeneBank Accession #
Relative Expression*
Name
GeneBank Accession #
Relative Expression*
h18S rRNA
X0325
****
m18S rRNA
K01364
****
hActß
NM_001101
***
mAct²
NM_007393
***
hB2m
NM_004048
***
mB2m
NM_009735
***
hGapdh
NM_002046
***
mGapdh
NM_008084
***
hPpia
NM_021130
***
mPpia
NM_008907
***
hRpl13a
NM_012423
***
mRpl13a
NM_009438
***
hRplp0
NM_001002
**
mHsp90ab1
NM_008302
**
hHprt1
NM_000194
**
mLdhal6b
NM_175349
**
hTfrc
NM_003234
**
mHprt1
NM_013556
**
hHsp90ab1
NM_007355
*
mGusb
NM_010368
*
hGusb
NM_000181
*
mTbp
NM_013684
*
hUbc
NM_021009
*
mTfrc
NM_011638
*

* Summary of the expression levels of genes in comparison with 18S rRNA using our sensitivity human or mouse reference cDNA and our proprietary SYBR green master mix.

Reaction Conditions:
95°C, 10 minutes -> (95°C, 15 seconds. -> 60°C, 30 seconds.) for 40 cycles -> melting curve.

Recommended Storage Condition: -20°C

Figure: Standard curve for housekeeping gene hprt1 expression at medium levels.

Additional information

OPTIONS

2 human genes, h18S rRNA and hActß, 100rxns, 2 high expression level hACtß, hGapdh; 2 medium expression level genes, hHprt1, hTfrc; 2 low expression level genes, hGusb, hUbc; 100rxns, All 12 human housing keeping genes at different expression levels, 100rxns, 2 mouse genes, m18S rRNA and mActß, 100rxns, 2 high expression level mACtß, mGapdh; 2 medium expression level genes, mHprt1, mHsp90ab1; 2 low expression level genes, mGusb, mTbp; 100rxns, All 12 mouse housekeeping genes at different expression levels, 100rxns

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