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IgG Purification using Protein A
IgG Purification using Protein A

Recombinant Protein A is an immunoglobulin-binding protein derived from the cell wall of the bacterium Staphylococcus aureus. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many of mammalian species, most notably IgGs. Protein A binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1. It does not react with human IgG3 or IgD, nor will it react to mouse IgM, IgA or IgE.
 
Name Cat# Description Price Qty
IgG Purification using Protein A
AIPA-100
Rabbit serum, 20 mg purified antibody
$472.00

Description:
Recombinant Protein A is an immunoglobulin-binding protein derived from the cell wall of the bacterium Staphylococcus aureus. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many of mammalian species, most notably IgGs. Protein A binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1. It does not react with human IgG3 or IgD, and mouse IgM, IgA or IgE.

Starting Material

Time

Rabbit serum

1-3 weeks

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Pyruvate Kinase I (pykF)
CE 10X Running Buffer (with EDTA)
Ammonium Persulfate (APS)
Pyruvate Kinase I (pykF) CE 10X Running Buffer (with EDTA) Ammonium Persulfate (APS)
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DpnI
RecA Protein, Tth
Firefly luciferase (photinus pyralis)
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Primers for Sequencing
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10X Thermal Chelator
Primers for Sequencing Kanamycin Sulfate 10X Thermal Chelator
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