JM109 Competent E. Coli

JM109 Competent E. Coli
Description

High yields, high efficiency, no heat shock necessary.
 
Name Cat# Description Price Qty
JM109 Competent E. Coli
JM-100
10x100μl (10 tubes), >1x10⁹
$110.00
JM109 Competent E. Coli
JM-196
96x50μl (96-tube rack), >1x10⁹
$320.00

Description:
High stability, high efficiency, no heat shock necessary. For Research Use Only.

Application:

  • Ideal for sub-cloning, single strained DNA, high quality plasmid preparation, and library construction
  • JM109 is a K strain bacterium that provides minimized recombination and aids in plasmid stability which results in high quality plasmid DNA preparation. The strain carries the hsdR17 genotype, which prevents cleavage of heter-ologous DNA by an endogenous endonuclease. JM109 strain supports Alpha-Complementation for blue/white screening for recombinant plasmids. The presence of the F' factor also allows growth of bacterio-phage M13 vectors for rescue of single stranded DNA. Transform efficiency is around 1.0 x 107 ~ 1.0 x 108 cfu/µg with pUC18 control DNA.

Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

Recommended reaction conditions:

  • Mix DNA with competent cell
  • Let stand on bench for 5 minutes
  • Direct plate

Genotype:
F' (traD36, proAB+, lacIq, lacZ-M15),endA1, recA1, hsdR17, (rk-, mk+), mcrA, supE44, e-gyrA96, relA1, -(lac-proAB)

Reference:

  1. Yanisch-Perron, C.; Vieira, J.; and Messing, J. Gene, (1985) 33, 103-119

Competent Cell Type:
Chemically

Transformation Efficiency (cfu/μg):
>1x109

Blue-Whiet Screening:
Yes

Strain:
K12

Reduces Recombination:
Yes

Cloning Methylated DNA:
No

Improves Plasmid Quality:
Yes

Preparing Unmethylated DNA:
Yes

T1 Phage Resistant:
No

RecA Deficient:
Yes

Manual & Protocols



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