JM109 Competent E. coli

Description

Description:
High stability, high efficiency, no heat shock necessary. For Research Use Only.

Application:

• Ideal for sub-cloning, single strained DNA, high quality plasmid preparation, and library construction
• JM109 is a K strain bacterium that provides minimized recombination and aids in plasmid stability which results in high quality plasmid DNA preparation. The strain carries the hsdR17 genotype, which prevents cleavage of heterologous DNA by an endogenous endonuclease. JM109 strain supports Alpha-complementation for blue/white screening for recombinant plasmids. The presence of the F’ factor also allows growth of bacteriophage M13 vectors for rescue of single stranded DNA. Transform efficiency is around >1×10⁹ cfu/µg with pUC18 control DNA.

Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.

Recommended reaction conditions:

• Mix DNA with competent cells
• Let stand on bench for 5 minutes
• Direct plate

Genotype:
F’ (traD36, proAB+, lacIq, lacZ-M15),endA1, recA1, hsdR17, (rk-, mk+), mcrA, supE44, e-gyrA96, relA1, -(lac-proAB)

Reference:

1. Yanisch-Perron, C.; Vieira, J.; and Messing, J. Gene, (1985) 33, 103-119

Competent Cell Type:
Chemically

Transformation Efficiency (cfu/μg):
>1×10⁹

Blue-Whiet Screening:
Yes

Strain:
K12

Reduces Recombination:
Yes

Cloning Methylated DNA:
No

Improves Plasmid Quality:
Yes

Preparing Unmethylated DNA:
Yes

T1 Phage Resistant:
No

RecA Deficient:
Yes

Additional information

Manual & Protocols

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