Pyruvate kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yields one molecule of pyruvate and one molecule of ATP.
T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma phosphate of ATP to the 5′-hydroxyl terminus of double-and single-stranded DNA, RNA and nucleoside 3′-monophosphate molecules (1).