Klenow Fragment (3´-> 5´ exo-)
Klenow Fragment (3´-> 5´ exo-)

Klenow Fragment, 3'->5' exo–, is the large fragment of E.coli DNA polymerase I with a molecular weight of 68kDa.

 
Name Cat# Size Concentration Price Qty
Klenow Fragment (3´→5´ exo–)
KPIM-100
10,000 units
50,000 U/ml
$128.75
Klenow Fragment (3´→5´ exo–)
KPIM-200
50,000 units
50,000 U/ml
$412.00
Klenow Fragment (3´→5´ exo–)
KPIM-OEM
Any Size
please inquire

Description:
Klenow Fragment, 3'->5' exo–, is the Large Fragment of E.coli DNA Polymerase I with a molecular weight of 68kDa. It exhibits 5'->3' polymerase activity, but lacks the 3'->5' exonuclease activities. The 3'->5' exonuclease activity of the enzyme is eliminated by mutations in the 3'->5' exonuclease active site.

Specific Activity:
5,000U/mg

Applications:

  • Random priming labeling
  • DNA sequencing by the Sanger dideoxy method
  • Second strand cDNA synthesis
  • Second strand synthesis in mutagenesis protocols

Supplied in:
20 mM Tris-HCl
100 mM NaCl
1.0 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25°C

Supplied with: 10x Blue Buffer

10x Blue Buffer:
500mM NaCl
100mM Tris-HCl
100mM MgCl2
10mM DTT
pH 7.9 @ 25 ºC

Unit Definition:
1 unit is defined as the amount of enzyme that will incorporate 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30min at 37°C.

Recommended Storage Condition: -20°C



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