Description:

• Chemically competent E. coli cells with high stability suitable for high efficiency transformation  For research use only.

• Parent of DH10B/TOP10 and derived strains

Applications:

• For cloning, sub-cloning and phage display, but cannot be used for reinfection
• Transform efficiency is >1.0x10⁸ cfu/µg with pUC19 control DNA

Genotype:  
F- Δ(ara-leu)7697 [araD139]B/r Δ(codB-lacI)3 galK16 galE15 λ- e14- mcrA0 relA1 rpsL150(StrR) spoT1 mcrB1 hsdR2(r-m+)

Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze

Competent Cell Type:
Chemically

Transformation Efficiency (cfu/μg):
>1.0x10⁸ cfu/µg with pUC19 control DNA

Blue-White Screening:
Yes, IPTG induction required

Strain:
K12

Reduces Recombination:
Yes

Cloning Methylated DNA:
Yes

Improves Plasmid Quality:
Yes

Preparing Unmethylated DNA:
Not suitable

T1 Phage Resistant:
No