Description
The MCMag PCR purification system utilizes solid-phase reversible immobilization (SPRI) paramagnetic bead technology for PCR amplicon purification. The MCMag beads are pre-formulated with an optimized buffer to selectively bind DNA fragments of 100 bp and larger. Excess salts, enzymes, primers and nucleotides can be removed through a simple washing procedure. The MCMag PCR Purification system is fully adaptable to automation.

Applications

• PCR
• Sequencing
• Fragment Analysis
• Genotyping
• Cloning
• Primer Walking

Material Supplied

• MCMag PCR Purification beads
• Store at 4°C upon arrival, for up to 18 months
• Shake the reagent well to a homogenous appearance before use

Specifications

Table 1. Available product sizes

Table 2. Number of PCR reactions purified with 96- and 384-well formats

Materials Not Supplied

• Reaction Plate
• McBead Magnetic Plate
• Plate Seals, Adhesive or Heat
• Wash Solution (70% ethanol)
• Elution Buffer
  • Water
  • Tris-Acetate (10 mM pH 8.0)
  • TE Buffer (10 mM Tris-Acetate pH 8.0, 1 mM EDTA