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MCNext™ DNA Sample Prep Kit
MCNext™ DNA Sample Prep Kit

The MCNext DNA Sample Prep Kit is a high performance, cost-effective sample prep solution for NGS using the Illumina platforms.

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Description

The MCNext DNA Sample Prep Kit is a high performance, cost-effective sample prep solution for NGS using the Illumina platforms. A bead-based, gel-free size selection workflow in only 90 min eliminates the need for mechanical fragmentation and agarose gels in size selection, the availability of pair-ends 96 indices facilitates multiplex high-throughput applications. The MCNext DNA Sample Prep Kit incorporates different flanking sequence additions by Dialatum™ transposome, resulting in fewer primer-dimers and a better signal/noise ratio in sequencing outcomes.

Fast Prep Workflow

  • Complete the protocol in 90 minutes
  • Enzymatic fragmentation without sonication
  • One-step fragmentation/tagging to save time  

Low Input and High Throughput

  • Maximum of 50 ng input DNA needed
  • Pre-mixed enzymes and nucleotides for multiplexed processing
  • Plate-based processing compatible for up to 96 samples per kit

Higher Coverage Rate and Full Compatibility

  • Directional transposome doubles the sequencing coverage
  • Works on all Illumina NGS platforms
  • All 96 Illumina indices included

MCNext DNA Sample Prep Kit figure 1.png
Figure 1. MCNext™ Sample Preparation Kit workflow takes 90 min to finish the sample prep.
MCNext DNA Sample Prep Kit figure 2.png
Figure 2. MCNext™ Sample Preparation Kit fragments and directionally tags DNA in a single step to boost sequencing coverage and signal strength. Subsequent limited-cycle PCR amplification adds dual indices to enable up to 96 libraries to be pooled and sequenced.

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