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McBead™ Endotoxin-Free Maxi Prep Plasmid Kit
McBead™ Endotoxin-Free Maxi Prep Plasmid Kit

MCLAB’s Maxi Plasmid Purification Kit offers a rapid, simple, and flexible method for isolating high quality, endotoxin removed supercoiled plasmid DNA from recombinant E. coli cultures. The kit features a filter syringe for rapid lysate clearing and magnetic particles for DNA capture.
 
Name Cat# Description Price Qty
McBead™ Endotoxin-Free Maxi Prep Plasmid Kit
EFX-100
10 preps
$110.00
McBead™ Endotoxin-Free Maxi Prep Plasmid Kit
EFX-200
25 preps
$200.00
McBead™ Endotoxin-Free Maxi Prep Plasmid Kit
EFX-300
100 preps
$700.00

Description

MCLAB’s Maxi Plasmid Purification Kit offers a rapid, simple, and flexible method for isolating high quality endotoxin-free* supercoiled plasmid DNA from recombinant E. coli cultures. The kit features a filter syringe for rapid lysate clearing and magnetic particles for DNA capture. Up to 1 mg** of plasmid DNA can be purified from a 150 mL overnight culture grown in Luria Broth (LB) medium.
An E. coli culture is harvested by centrifugation and treated to a modified alkaline-SDS lysis procedure. Following the lysate clearing, DNA is selectively adsorbed to the magnetic particles in the presence of high salts and alcohol. Contaminants are removed by two washing steps. The bound DNA is then eluted and subjected to isopropanol precipitation as a final polishing step.
The recovered product is of high-quality and predominantly supercoiled plasmid DNA that is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation and transfection.


Features

• Fast (prep in 30 minutes)
• High quality (supercoiled plasmid DNA ready for all applications)
• High yield (up to 1 mg per prep) 
• In vivo study ready (endotoxin free*) 
• No phenol/chloroform extraction or alcohol precipitation required

* MCLAB’s endotoxin free standard is <0.1 EU/ug plasmid DNA.
** Actual yields may vary based on the bacterial strain, plasmid and culture medium used.

Components

Components

EFX-100

EFX-200

EFX-300

Resuspension Buffer

100 ml

250 ml

1000 ml

Lysis Buffer

100 ml

250 ml

1000 ml

Neutralization Buffer

100 ml

250 ml

1000 ml

ETR Binding Buffer

180 ml

450 ml

1800 ml

Magnetic Beads

10 ml

25 ml

100 ml

Wash 1 Buffer

200 ml

450 ml

2000 ml

Filter Syringe

10 pc

25 pc

100 pc

RNase A

40 ml

100 ml

400 ml

Endotoxin-Free Elution Buffer

30 ml

75 ml

300 ml

 

RNAse Inhibitor
Figure 1.This is a Maxiprep of a 6KB plasmid comparison between MCLAB and Supplier Q (ion-exchanged column-based). Based on 260/280 spectrophotometer, the same amount of DNA is loaded onto a 1% agarose gel from MCLAB and Supplier Q.  Note: 1 µg DNA of Supplier Q contains high impurities such as genomic DNA, smears of non-specific DNA fragments, and less of the specific plasmid DNA as oppose to MCLAB.

RNAse Inhibitor
Figure 2.This is a Maxiprep of PCDNA plasmid comparison between MCLAB and Supplier Q (ion-exchanged column-based).

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