Description
MCLAB’s McExtract Gel Extraction Kit offers specially designed paramagnetic beads and buffers for rapid purification of DNA fragments from agarose gels. DNA ranging from 70 bp to 10 kb is extracted from molten gels using a rapid binding and magnetically separating procedure. Captured DNA is then washed and eluted. The procedure provides faster and easier way of DNA gel purification through magnetic beads and magnets rather than the use of column centrifuge. It can be easily transitioned to large-scale purification or automation.

Applications

DNA purified with the Gel Extraction Kit can be used directly in most molecular biology applications, including:

• Subcloning
• Sequencing
• Labeling & DNA hybridization
• DNA concentration

Highlights

• Fast and easy purification of DNA fragments from an agarose gel using MCLAB’s magnetic beads
• No need for centrifugation
• A single kit fits from small to large scale of extractions, and can be easily adapted to an automation format

Experimental Data

Figure 1: Comparison of DNA fragments from agarose gel extracted with McExtract Gel Extraction Kit and competitor Q’s kit
 

Gel on the left:
L: 1 Kb ladder
1: DNA product before gel purification
MC: 2 kb DNA purified with McExtract Gel Extraction Kit
Q: 2 kb DNA purified with competitor Q’s kit
Gel on the right:
L: 1 Kb ladder
1: DNA product before gel purification
MC: 1 kb DNA purified with McExtract Gel Extraction Kit
Q: 1 kb DNA purified with competitor Q’s kit

Kit Component