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NGS Miscellaneous

NGS Miscellaneous

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ATP Sulfurylase Yeast
Adenosine 5' Triphosphate Sulfurylase is purified from E.coli containing Yeast adenosine 5' Triphosphate Sulfurylase gene.
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DNA Homopolymeric Tailing Super Mix Kit
DNA Homopolymeric Tailing Super Mix provides qualified reagents for the addition of homopolymer tails to the 3' ends of DNA with terminal deoxynucleotidyl transferase (TdT).
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Fpg, <i>E. coli</i>

Fpg (also known as Formamidopyrimidine DNA glycosylase, Mut M, FAPY DNA Glycosylase, and 8-oxoguanine DNA glycosylase) participates in the base-excision (BER) pathway of DNA repair enzymes and acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic/apyrimidinic (AP site). The AP-lyase activity cleaves both the 3′ and 5′ phosphodiester bonds at the AP site, producing a 1 base gap in the DNA and 3′ and 5′ phosphate termini. Bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil (1,2).

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QuantumScript™ HD Reverse Transcriptase
QuantumScriptTM HD Reverse Transcriptase is a unique mutant version with increased thermostability and reduced RNase H activity. It can be used to synthesize first strand cDNA by reverse transcription (RT) at higher temperatures than other types of Moloney Murine Leukemia Virus (M-MuLV) RT and to reach higher cDNA yields for difficult RNA transcription.
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QuantumScript™ Reverse Transcriptase
Universal Reverse Transcriptase is a mutation with reduced RNase H activity and increased thermostability.
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RCA DNA Amplification Kit
RCA DNA amplification kits are novel products developed specifically to prepare templates for DNA sequencing. The RCA method utilizes bacteriophage phi29 DNA polymerase to exponentially amplify single- or double-stranded circular DNA templates by rolling circle amplification (RCA). This isothermal amplification method produces microgram quantities of DNA from picogram amounts of starting material in a few hours.
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RNA Poly(A) Tailing Kit

The RNA Poly(A) Tailing Kit provides a highly pure and active enzyme and other reagents for quickly and easily adding a poly(A) tail to the 3' end of any RNA.

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RNAse Inhibitor
RNAse Inhibitor is an acidic, 52 kDa protein that is a potent non-competitive inhibitor of pancreatic-type ribonucleases such as RNase A, RNase B, and RNase C.
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SmartRT™ Reverse Transcriptase Kit

SmartRTTM Reverse Transcriptase is an engineered MMLV RT that improves the enzyme’s thermostability, reduces RNase H activity and its cDNA synthesis ability. The enzyme also has a terminal transferase activity, where it adds a few extra nucleotides to the end of the synthesized cDNA.  With a 3’ modified oligo dT primer and a 5’  SMART universal oligo containing a terminal complementation to nucleic acids at the 3’  end of the first-strand cDNA, the SmartRTTM reverse transcriptase will produce RACE ready full-length cDNA. 

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Human Genomic DNA
Human Genomic DNA is used in DNA typing, DNA analysis, human identity testing, and tissue culture strain identification
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Gel/PCR DNA Isolation System
Gel/PCR DNA Isolation System has been developed to isolate DNA from agarose gel, PCR products, and other enzymatic reactions.
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DNA Storage Buffer (Low Concentration)
Low concentration solutions of DNA are prone to DNA degradation or other loss. It is critical that optimal methods are employed for DNA suspension and long-term storage.
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RNA Storage Buffer

MCLAB's RNA Storage Buffer, with optimized reagents and pH value, provides better preservation and greater RNA stability for valuable RNA samples than the standard EDTA and TE Buffer or just water.

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