NGS Miscellaneous

Fpg (also known as Formamidopyrimidine DNA glycosylase, Mut M, FAPY DNA Glycosylase, and 8-oxoguanine DNA glycosylase) participates in the base-excision (BER) pathway of DNA repair enzymes and acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic/apyrimidinic (AP site). The AP-lyase activity cleaves both the 3′ and 5′ phosphodiester bonds at the AP site, producing a 1 base gap in the DNA and 3′ and 5′ phosphate termini. Bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil ^(1,2).

The RNA Poly(A) Tailing Kit provides a highly pure and active enzyme and other reagents for quickly and easily adding a poly(A) tail to the 3' end of any RNA.

SmartRT^TM Reverse Transcriptase is an engineered MMLV RT that improves the thermostability of the enzyme, reduces RNase H activity and its cDNA synthesis ability. The enzyme also has a terminal transferase activity, where it adds a few extra nucleotides to the end of the synthesized cDNA.  Together with a 3’ modified oligo dT primer and a 5’  SMART universal oligo, which contains a terminal complementation to nucleic acids at the 3’  end of the first-strand cDNA, the SmartRT^TM reverse transcriptase produces RACE ready full-length cDNA.