
Also Available FastSep™ High Performance NTA Resins
Description
FastSep high performance resins and chromatography cartridges - Numerous ligand-metal ion combinations Application - For His-tagged protein purification A his-tag, or polyhistidine tag, is a string of histidine residues at either the N or C terminus of a recombinant protein. Such a string can have 4 to 10 residues, with 6 being the most common a hexahistidine tag. Recombinant proteins can have more than one hexahistidine tag. NTA and IDA are the two most widely used ligands in IMAC. NTA is a tetravalent ligand with coordination number 4 and therefore stronger coordination of metal ions, while IDA is a trivalent ligand with coordination number 3. Generally, NTA gives higher binding specificity and lower metal ion leaching, while IDA shows more non-specific binding and increased metal ion leaching. IDA can have higher metal ion loading density and usually requires much lower imidazole concentration in eluent. Specifications
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