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NGS Servcies
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The custom 5’ adenylation service is designed for generating 5’ pre-adenylated DNA/RNA oligonucleotides. Adenylated oligos with a pyrophosphate linkage are substrates of T4 RNA ligase in the absence of ATP, which can significantly reduce undesired self-ligation and other side products.
Whole genome sequencing reveals the complete DNA make-up of an organism, enabling researchers to better understand variations both within and between species, and to differentiate organisms with a precision that other technologies do not allow. For de novo whole-genome sequencing, the unparalleled raw read accuracy of Illumina NGS technology provides high quality, long contig assemblies.
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The diagnosis of mitochondrial disorders is challenging because of the clinical variability and genetic heterogeneity. Mitochondrial sequencing with NGS technologies provides a robust high-throughput platform for comprehensive detection and analysis of mitochondrial mutations.
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16S ribosomal RNA (rRNA) sequencing is a common amplicon sequencing method used to identify and compare bacteria present within a given sample. 16S rRNA gene sequencing is a well-established method for studying phylogeny and taxonomy of samples from complex microbiomes or environments that are difficult or impossible to study.
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The exome represents less than 2% of the human genome, but contains ~85% of known disease-causing variants, making whole-exome sequencing a cost-effective alternative to whole-genome sequencing. With exome sequencing, you can investigate the protein coding regions of the genome when sequencing an entire genome is not practical or necessary. It can efficiently identify variants across a wide range of applications, including population genetics, genetic disease, and cancer studies.
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mRNA sequencing (mRNA-Seq) delivers unbiased and unparalleled information about the transcriptome without probe design. Strand information provides increased confidence in transcript annotation, particularly for non-human samples, and may increase the percentage of alignable reads, reducing sequencing costs per sample. Strand orientation also enables the detection of antisense expression, providing visibility to regulatory relationships that would otherwise be missed.
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Small RNA populations can vary significantly between different tissue types and species. As next-generation sequencing enables tremendous output with unprecedented sensitivity and dynamic range, small RNA sequencing has been rapidly adopted for both small RNA discovery and profiling applications
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