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T7 Exonuclease is similar to Lambda Exonuclease in that it catalyzes the stepwise hydrolysis of duplex DNA from the 5' termini liberating 5' mononucleotides. However, unlike Lambda Exonuclease, the enzyme has low processivity and it will remove both 5-hydroxyl and 5-phosphcryl termini.
Modified Enzyme. catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate.
FEN1 (AFU) is a highly purified recombinant flap endonuclease-1 (FEN-1) protein from the E. coli containing FEN1 Gene of hyperthermophilic Archaea strain, Archaeoglobus fulgidus. The FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis.
Exonuclease III is a 3′→ 5′ exonuclease which acts by digesting one strand of a dsDNA duplex at a time or digesting the RNA strand of an RNA-DNA heteroduplex (1).
Exonuclease I cleaves single-stranded DNA in the 3′→ 5′ direction, releasing 5′ -mono/di-nucleotides and leaving double-stranded DNA molecules and the 5'-terminus intact.
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