Description:
The 2X Ori-Master Mix is a premixed, ready-to-use solution containing a high-fidelity DNA polymerase, ori specific primers, dNTPs, and MgCl₂. The Master Mix can produce linear copies of bacterial plasmids from a single colony. The reaction mixture and conditions are optimized regardless of the plasmid copy number. Amplified products can be directly sequenced without the need for purification.

Applications:

• Colony PCR
• DNA sequencing
• Restriction Digestion

Features:

• Fast reaction : less than 2hrs from a colony to its DNA products, comparing to 16-24 hours for a miniprep process
• Good yield: a 25 μl reaction yields up to 1 μg DNA
• Easy to use: Ori-specific primers are included and the PCR protocol is optimized. Just add the template and water.
• Long amplification fragments: up to 10 Kb
• High throughput capability: 96 or 384 well plate compatible

Experimental Data :