2X Ori-Master Mix
2X Ori-Master Mix

The 2X Ori-Master Mix is a premixed, ready-to-use solution containing a high-fidelity DNA polymerase, ori specific primers, dNTPs, and MgCl2.

 
Name Cat# Description Price Qty
2X Ori-Master Mix
OMM-100
2.5 ml for 200 rxn
$204.97
2X Ori-Master Mix
OMM-200
10 ml for 800 rxn
$614.91
2X Ori-Master Mix
OMM-300
100 ml for 8000 rxn
$4944.00

Description:
The 2X Ori-Master Mix is a premixed, ready-to-use solution containing a high-fidelity DNA polymerase, ori specific primers, dNTPs, and MgCl2. The Master Mix can produce linear copies of bacterial plasmids from a single colony. The reaction mixture and conditions are optimized regardless of the plasmid copy number. Amplified products can be directly sequenced without the need for purification.

Applications:

  • Colony PCR
  • DNA sequencing
  • Restriction Digestion

Features:

  • Fast reaction : less than 2hrs from a colony to its DNA products, comparing to 16-24 hours for a miniprep process
  • Good yield: a 25 μl reaction yields up to 1 μg DNA
  • Easy to use: Ori-specific primers are included and the PCR protocol is optimized. Just add the template and water.
  • Long amplification fragments: up to 10 Kb
  • High throughput capability: 96 or 384 well plate compatible

Experimental Data :



Figure. Colony PCR Using 2X Ori-Master Mix
M:1 Kb DNA ladder (NEB). 1&2:PCR product of ~4Kb plasmids.

Manual & Protocols



MSDS & Certificates

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