Magnesium is a cofactor for thermostable DNA polymerases. This patent-pending technology relates to thermally reversible chelators of magnesium, thus a hot-start-cold-stop PCR is achieved.
High quality deoxynucleotide triphosphate (dNTPs) are functionally tested in long PCR to be PCR qualified, and meet or exceed the criteria for high-quality sequencing with Thermo Sequenase DNA polymerase.
