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PDI1 (yeast)
PDI1 (yeast)

Recombinant yeast PDI is produced in E.coli is as single, non-glycosylated, polypeptide chain containing 503 amino acids and having a molecular mass of 62.4 kDa. The PDI has a 6His-tag at N-terminus and is purified by proprietary chromatographic techniques.
 
Name Cat# Size Concentration Price Qty
PDI1 Yeast (Liquid)
PDI-100
10 mg
10 mg/ml
$120.00
PDI1 Yeast (Liquid)
PDI-200
40 mg
10 mg/ml
$360.00
PDI1 Yeast (Lyophilized)
PDI-300
10 mg
10 mg/ml
$120.00
PDI1 Yeast (Lyophilized)
PDI-400
40 mg
10 mg/ml
$360.00
PDI1 Yeast
PDI-OEM
Any Size
please inquire

Description:
Recombinant yeast Protein Disulfide Isomerase is produced in E. coli as a single, non-glycosylated, polypeptide chain, containing 503 amino acids and having a molecular mass of 62.4 kDa.The PDI has a 6His-tag at N-terminus and is purified by proprietary chromatographic techniques.
Recombinant Yeast Protein Disulfide Isomerase is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. Recombinant PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro.

Source:  E. coli

Recommended Storage Condition: -20ºC

Physical Appearance:
Sterile filtered liquid form or lyophilized powder

Formulation:
The PDI protein (10mg/ml)solution was lyophilized from PBS pH 7

Solubility:
It is recommended to reconstitute the lyophilized PDI in sterile 18MΩ-cm H2O (not less than 100µg/ml), which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Protein Disulfide Isomerase, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Human PDI should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:
Greater than 99.0% as determined by RP-HPLC.

Reductase Activity:
0.001 650nm/ min-2. By measuring the turbidity increase at 650 nm due to insulin reduction (Holmgren, A. (1979) J. Biol. Chem. 254, 96279632). The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time (Mart´nez-Galisteo, E., Padilla, C. A., Garcia-Alfonso, C., Lo´pez-Barea, J., and Barcena, J. A. (1993) Biochimie (Paris) 75, 803809).

Isomerase Activity:
0.5 µmol active RNase A min-1 µmol PDI-1. According to the re-activation of reduced and denatured RNase A (Lyles, M. M. and Gilbert, H. F. (1991) Biochemistry 30, 613-619).

Manual & Protocols



COA ( Certificate of Analysis )



MSDS & Certificates

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