Description:
Phi29 DNA Polymerase is responsible for the replication of the Bacillus Subtilis phage phi29⁽¹⁾. The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand displacement activity⁽²⁾ and a 3' -> 5' proofreading exonuclease function⁽³⁾.

Specific Activity: 100,000U/mg

Applications:

• Catalyzes the removal of 5'-mononucleotides from duplex DNA
• Replication requiring a high degree of strand displacement and/or processive synthesis
• High fidelity replication at moderate temperatures

Source:
A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29

Supplied in:
10 mM Tris-HCl
100 mM KCl
0.1 mM EDTA
1 mM DTT
0.5% Tween-20
0.5% NP-40
50% Glycerol
pH 7.4 @ 25°C

Supplied with:
10x phi29 DNA Polymerase Reaction Buffer

10x phi29 DNA Polymerase Buffer
500 mM Tris-HCl
100 mM (NH₄)₂SO₄
40 mM DTT
100 mM MgCl₂
pH 7.5 @ 25°C

Unit Definition:
1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dTTP into acid insoluble material in 10 minutes at 30°C.

Recommended Storage Condition: -20°C

References:
1. Blanco, L. and Salas, M. (1984) Proc. Natl. Acad. Sci. USA, 81, 5325-5329.
2. Blanco, L. et al. (1989) J. Biol. Chem., 264, 8935-8940.
3. Garmendia, C. et al. (1992) J. Biol. Chem., 267, 2594-2599.