Description:
Phi29 DNA Polymerase is responsible for the replication of the Bacillus Subtilis phage phi29(1). The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand displacement activity(2) and a 3' -> 5' proofreading exonuclease function(3).
Specific Activity: 100,000U/mg
Applications:
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Catalyzes the removal of 5'-mononucleotides from duplex DNA
-
Replication requiring a high degree of strand displacement and/or processive synthesis
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High fidelity replication at moderate temperatures
Source:
A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29
Supplied in:
10 mM Tris-HCl
100 mM KCl
0.1 mM EDTA
1 mM DTT
0.5% Tween-20
0.5% NP-40
50% Glycerol
pH 7.4 @ 25°C
Supplied with:
10x phi29 DNA Polymerase Reaction Buffer
10x phi29 DNA Polymerase Buffer
500 mM Tris-HCl
100 mM (NH4)2SO4
40 mM DTT
100 mM MgCl2
pH 7.5 @ 25°C
Unit Definition:
1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dTTP into acid insoluble material in 10 minutes at 30°C.
Recommended Storage Condition: -20°C
References:
1. Blanco, L. and Salas, M. (1984) Proc. Natl. Acad. Sci. USA, 81, 5325-5329.
2. Blanco, L. et al. (1989) J. Biol. Chem., 264, 8935-8940.
3. Garmendia, C. et al. (1992) J. Biol. Chem., 267, 2594-2599.
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