Protein Purification solutions

Protein Purification solutions

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50% IGEPAL CA-630 (NP-40)
Reported use in all phases of detergent compounding and aqueous processing in the textile and paper industries, in industrial metal cleaners, acid cleaners, floor cleaners, detergent sanitizers and waterless hand cleaners.
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50% Triton x-114
TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C). At temperatures above the cloud point, detergent solutions separate into aqueous and detergent-enriched phases. This makes Triton X-114 particularly useful in separating lipophilic proteins from hydrophilic proteins.2,3 For example, HMG-CoA reductase was recovered quantitatively in the aqueous phase of a biphasic system formed by TRITON X-114 at 30 °C.
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7M Guanidine Hcl Solution
MCLAB’s 7M Guanidine-HCl Solution is a ready-to-use solution of guanidine hydrochloride, that can be easily diluted and pH-adjusted to any concentration below 7M. In addition to increasing solubility of hydrophobic molecules, guanidine is a general protein denaturant, unfolding proteins and altering their structure. This can cause some proteins to be irreversibly altered upon interaction with guanidine solutions and may lose their binding function.
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Acetylated Bovine Serum Albumin (BSA)
Acetylated BSA is used as a carrier protein and as an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is vital. The acetylation process inactivates any trace of active nuclease activity. While the acetylation process does change BSA's binding characteristics and makes it inhibitory in PCR reactions, it is normally added to restriction digests.
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Ammonium Sulfate
Ammonium Sulfate.(NH4)2SO4, is an inorganic salt with a number of commercial uses.
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Antifoam 204
Contains 100% active components and is a mixture of organic non - silicone polypropylene based polyether dispersions. May be sterilized repeatedly.
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Brij-35 (30% Solution)
Non-ionic Detergent Brij-35 (30% Solution), DB35-100 (950ml, $66.00)
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CHAPS
CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate ) is a zwitterionic detergent used to solubilize proteins. It is used as a non-denaturing solvent in the process of protein purification and is especially useful in purifying membrane proteins, which are often sparingly soluble or insoluble in aqueous solution due to their native hydrophobicity. CHAPS can also be used in conjunction with nonionic detergents such as Triton X-100.
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CHAPSO
CHAPSO(3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate) is a zwitterionic detergent used to solubilize proteins. Due to its low light absorbance in the ultraviolet region of the electromagnetic spectrum, CHAPSO is useful for researchers monitoring ongoing chemical reactions or protein-protein binding with UV/Vis spectroscopy
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TWEEN 20
TWEEN 20 helps to prevent non-specific antibody binding in enzyme immunoassay as a washing agent (e.g. Western blots and ELISAs). In this major application, it is dissolved in Tris-Buffered Saline or Phosphate buffered saline at dilutions of 0.05% to 0.5% v/v. These buffers are used for washes between each immunoreactions, to remove unbound immunologicals, and eventually for incubation solutions of immunoreagents (labeled antibodies) to reduce unspecific background.
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Ultrapure Bovine Serum Albumin (BSA)
Bovine Serum Albumin (BSA) has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions. MCLAB’s Ultrapure BSA is a "non-acetylated" BSA, pure enough to use when DNA or RNA integrity is essential. It has been tested for DNase, RNase, endonuclease, protease, peroxidase, and alkaline phosphatase activity, and assayed for fluorescence background.
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