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RNA-Seq Library Construction Kit
RNA-Seq Library Construction Kit

MCLAB's RNA-Seq Library Construction Kit is a highly efficient library construction kit for preparing single, paired-end or multiplexed cDNA libraries for high-throughput sequencing.
 
Name Cat# Description Price Qty
RNA-Seq Library Construction Kit
NGRR-100
8 reactions
$620.00
RNA-Seq Library Construction Kit
NGRR-200 
24 reactions
$1480.00
RNA-Seq Library Construction Kit
NGRR-300 
48 reactions
$2360.00

Description:
MCLAB's RNA-Seq Library Construction Kit is a highly efficient library construction kit for preparing single, paired-end or multiplexed cDNA libraries for high-throughput sequencing. This kit can be used to convert RNA transcripts into cDNA whole transcriptome libraries or small RNA libraries for next-generation sequencing analysis using Illumina® GAIIxTM, HiSeqTM 2000 and MiSeq® sequencing platforms. With preserving the complexity of sequencing libraries, the protocol is quicker than the standard method and could be used routinely for RNA sequencing through the Illumina® platform.

Function:
The superior function of MCLAB's RNA-Seq Library Construction Kit depends on our proprietary enzyme systems. Unique improvement to each key enzyme at specific steps in the library construction workflow increases sensitivity, flexibility and speed for next-generation sequencing. The target RNA fragments can be tagged with linkers at both ends by RNA ligases (Cat# T4RL1-100 and T4RL2T-100). Corresponding cDNA can be synthesized by Universal Reverse Transcriptase (Cat# SSII-100). The cDNA library can be enriched by PCR with high fidelity Pfu DNA Polymerase (Cat# AD-200). After gel- or beads-based purification for size selection, final cDNA libraries can hybridize directly to the oligonucleotides on the flow cell surface for cluster generation and sequencing thereafter.

Features:
- Simplified workflow, reducing hands-on time
- Optimized reaction conditions, increasing sensitivity
- Straightforward protocol minimizing prior experience needed
- Strand-specific sequencing data
- Cost-effective with all key enzymes
- High flexibility: multiplex samples and automation friendly
- Functionally validated with Illumina® GAIIxTM, HiSeqTM 2000 and MiSeq® sequencing platforms

List of Components:

Sufficient reagents are supplied in the MCLAB RNA-Seq Library Construction Kit (Illumina®-compatible) to prepare cDNA libraries from 8 samples for high-throughput sequencing through the Illumina platform.

Upon receipt of the kit, immediately store the components at –20 °C in a freezer without a defrost cycle.

Box 1:

Fragmentation Solution               27 µl

Fragmentation Enzyme Mix          36 µl

Linker-A                                      13.5 µl

Linker Enzyme Mix-A                   18 µl

Linker Addition Buffer                 18 µl

Reaction Enhancer                       13.5 µl

Linker-B                                      13.5 µl

Linker Enzyme Mix-B                   13.5 µl

 

Box 2:

RT Primer                                    22.5 µl

RT Mix                                        22.5 µl

PCR Mix                                      225 µl

Primer-1                                      18 µl

Library Elution Buffer                   1.5 mL

 

Box 3:

Index Primer 1                              18 µl

Index Primer 2                              18 µl

Index Primer 3                              18 µl

Index Primer 4                              18 µl

Index Primer 5                              18 µl

Index Primer 6                              18 µl

Index Primer 7                              18 µl

Index Primer 8                              18 µl


Recommended Storage Conditions: -20°C

Shipping Condition: Dry ice

The Q&A for this product will be available soon.
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