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RNase H, Tth
RNase H, Tth

RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules.
 
Name Cat# Size Concentration Price Qty
RNase H, Tth
RNHT-100
2,500U
$150.00
RNase H, Tth
RNHT-200
10,000U
$480.00
RNase H, Tth
RNHT-OEM
Any Size
please inquire

Description
RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules. RNase H digestion produces ribonucleotide molecules with 5'-phosphate and 3'-hydroxyl termini. RNAse H is nearly inactive against single or double-stranded RNA molecules.
RNase H from the thermophilic eubacterium Thermus thermophilus HB8 is thermostable, has optimal activity above 65°C and is active up to 95°C, making it useful for a broad range of applications.

Applications

  • Removal of poly(A) tails of mRNA hybridized to poly(dT)
  • Removal of mRNA during second strand cDNA synthesis

Source:
A recombinant E. coli strain carrying the RNAse H gene from the thermophilic eubacterium Thermus thermophilus HB8.

Supplied in:
20 mM Tris-HCl
100 mM KCl
10 mM MgCl2
0.1 mM EDTA
0.1 mM DTT
50% Glycerol
pH 7.9 @ 25°C

Supplied with:
10x RNAse H Buffer

10x RNAse H Buffer:
500 mM Tris-HCl
750 mM KCl
30 mM MgCl2
100 mM DTT
pH 8.3 @ 25°C

Unit Definition:
1 unit is defined as the amount of enzyme that will hydrolyze 1 nmol of RNA from an 3H-labeled DNA:RNA hybrid molecule into acid-soluble material in 20 minutes at 37°C.

Recommended Storage Condition: -20°C

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