RNase Inhibitor, Murine
RNase Inhibitor, Murine

RNase Inhibitor, Murine is a 50 kDa recombinant protein of murine origin.
 
Name Cat# Size Concentration Price Qty
RNAse Inhibitor
RNIN-100
20,000 units
40,000 U/ml
$128.75
RNAse Inhibitor
RNIN-200
40,000 units
40,000 U/ml
$206.00
RNAse Inhibitor
RNIN-OEM
Any Size
please inquire

Description:
RNase Inhibitor, Murine is a 50 kDa recombinant protein of murine origin. The inhibitor specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H. Recombinant murine RNase inhibitor has significantly improved resistance to oxidation compared to the human/porcine RNase inhibitors, and is stable at 1mM DTT.

Application:
RNase Inhibitor can help prevent RNase degradation in any application where RNases could cause problems.

  • protect mRNA during cDNA synthesis reactions, RT-PCR  and LAMP reaction
  • in vitro transcription reactions (SP6, T7, or T3 system).
  • inhibit RNases during RNA isolation and purification.
Source:
An E. coli strain that carries the Ribonuclease Inhibitor gene from mouse.

Supplied in:
20 mM Hepes-KOH
50 mM KCl
8 mM DTT
50% Glycerol
pH 7.5 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme required to inhibit 50% the hydrolysis of cytidine 2',3'-cyclic monophosphate by 5 ng of RNAse A.(1)

Recommended Storage Condition: -20°C

Reference:
1. Blackburn, P., 1979. Ribonulcease Inhibitor from Human Placenta: Rapid Purification and Assay. The Journal of Biological Chemistry, Vol. 254, No. 24 pp 12484-12487.

Manual & Protocols



COA ( Certificate of Analysis )



MSDS & Certificates

RNAse Inhibitor
Figure 1. RNase Inhibitor Activity test by SpectraMax M5 system.
Comparing to benchmark’s and paralleled negative control, results show MCLAB’s RNAse Inhibitor are functionally equivalent with benchmark’s product.

RNAse Inhibitor
Figure 2. RNAse Inhibitor functional test.
Gel electrophoresis after RT-PCR shows MCLAB’s RNAse Inhibitor is functionally equivalent with benchmark’s product.

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